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Rapid generation of patient-derived single cells from solid tumors

Recent developments have the potential to bring personalized medicine one step closer to being utilized in clinical settings. Traditional sample preparation techniques, however, are not robust or reproducible.

In this study, the TissueGrinder, a revolutionary mechanical semi-automated benchtop device, is tested for cell isolation from surgically removed tumor tissues. It can extract cells from tissue in a very quick and enzyme-free manner.
 
 
 
Microscopy image of human connective tissue cells cultured in vitro for primary cell isolation.
Close-up of a pipette dispensing pink liquid into a microtube plate for cell isolation and sample preparation in a biomedical lab.

Our findings demonstrate that TissueGrinder can handle solid tumor tissues more quickly, efficiently, and successfully than the explant technique that is typically used:

Light blue double arrow symbol pointing right, representing speed, forward motion, or process acceleration.

TissueGrinder enables isolation of cells from various tumor tissues.

Light blue double arrow symbol pointing right, representing speed, forward motion, or process acceleration.

Tumor tissue processed using the TissueGrinder generated more heterogeneous cell populations.

Light blue double arrow symbol pointing right, representing speed, forward motion, or process acceleration.

Samples processed with the TissueGrinder were accessible for cell analysis about one week earlier.

TissueGrinder enzyme-free tumor tissue dissociation for single-cell isolation compared to explant method.

Reference: Scheuermann S, Lehmann JM, Ramani Mohan R, Reißfelder C, Rückert F, Langejürgen J and Pallavi P (2022) TissueGrinder, a novel technology for rapid generation of patient-derived single cell suspensions from solid tumors by mechanical tissue dissociation. Front. Med. 9:721639. doi: 10.3389/fmed.2022.721639

The generated single cell suspension can be employed to create tissue models such as spheroids, micro tissues, organoids and cell printed systems. New cell lines can easily be established for molecular cell characterization and drug screenings.

An example of a single cell suspension of a primary liver tumor processed with the TissueGrinder

Microscopy image of primary liver tumor cells cultured after tissue dissociation into a single-cell suspension.
Microscopy image of liver tumor-derived cells in culture after tissue dissociation.
Close-up of TissueGrinder tube components with transparent grinding inserts and blue cap for tissue dissociation.
TissueGrinder benchtop device for automated mechanical tissue dissociation in laboratory workflows.
Hand holding a 50 ml centrifuge tube with blue cap and pink solution for tissue processing.

Sounds too good to be true?

Then contact us to arrange a test run with your own samples!

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