It facilitates a standardized sample preparation with subsequent filtration in a closed, sterile system, based on standard labware. A benchtop-device with four grinding slots allows customizable process control for the mechanical dissociation, yielding high quality single cell suspensions from fresh or frozen or even FFPE tissue samples, which can be used for a multitude of applications. By employing an ideal combination of shearing and cutting forces, highly viable cells can be obtained for cytometry, further cytological and molecular analysis or cultivation.