Enzyme-free tissue dissociation in just a few minutes ...

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employing a simple, yet gentle process ...

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integrated in standard labware, including cell-filtration ...

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allowing contamination-free handling of your precious tissue samples ...

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featuring customizable protocols ...

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resulting in a high yield of unadulterated viable cells ...

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maintaining surface markers

TissueGrinder benchtop device
TissueGrinder consumable
Hepatocytes after dissociation
Tissue Dissociation Workflow

© Fraunhofer IPA

The tissue dissociation technology TissueGrinder...

…was developed at the Fraunhofer Institute for Manufacturing Engineering and Automation in Mannheim.

It facilitates a standardized sample preparation with subsequent filtration in a closed, sterile system, based on standard labware.

A benchtop-device with four ports allows customizable process control for mechanical dissociation with special grinding consumables, yielding high quality single cell suspensions from fresh or frozen or even FFPE tissue samples, which can be used for a multitude of applications.

By employing an ideal combination of shearing and cutting forces, highly viable cells can be obtained for cytometry, further cytological and molecular analysis or cultivation.

Fraunhofer IPA TissueGrinder Benchtop

© Fraunhofer IPA

TissueGrinder-Benchtop with Software and single-use Consumables
Hepatocytes after dissociation

The tissue dissociation technology TissueGrinder...

…was developed at the Fraunhofer Institute for Manufacturing Engineering and Automation in Mannheim.

It facilitates a standardized sample preparation with subsequent filtration in a closed, sterile system, based on standard labware.

A benchtop-device with four ports allows customizable process control for mechanical dissociation with special grinding consumables, yielding high quality single cell suspensions from fresh or frozen or even FFPE tissue samples, which can be used for a multitude of applications.

By employing an ideal combination of shearing and cutting forces, highly viable cells can be obtained for cytometry, further cytological and molecular analysis or cultivation.

Hepatocytes after dissociation
Fraunhofer IPA TissueGrinder Benchtop

© Fraunhofer IPA

TissueGrinder-Benchtop with Software and single-use Consumables
Play Video

© Fraunhofer IPA

Get an impression on how straight forward and easy it is to generate a single cell suspension from a tissue sample with the TissueGrinder

TissueGrinder consumable unit with integrated filtration in standard labware
Single cell suspension after centrifugation

© Fraunhofer IPA

The TissueGrinder is able to dissociate a great variety of tissue types in different conditions – healthy, tumorous, fresh, frozen or even FFPE.

Generating single cell suspensions from tissue samples is highly individual. With easily adaptable protocols, the TissueGrinder accomodates specific customizations for each application.

The mechanical process of the TissueGrinder matches or even outperforms the best enzymatic protocols in terms of cell yield and viability.

The mechanical process of the TissueGrinder matches or even outperforms the best enzymatic protocols in terms of cell yield and viability

Scientific references demonstrate
the utility of the TissueGrinder technology

The TissueGrinder from Fast Forward Discoveries quite literally saved my project. I had exhausted all possible options for dissociating fixed cancer cell spheroids for subsequent single-cell signalling studies, when I came across this product. I could hardly believe my eyes when I saw the perfect single-cell suspension generated by my demo TissueGrinder after just 3 minutes of the standard “Harsh” protocol (and with no enzymes!).
It is now an integrated part of my single-cell signalling workflows, and I dare say some of my key discoveries over the last year would not have been possible without this equipment.”
Ralitsa Madsen – UCL, Sir Henry Welcome Postdoctoral Fellow:
https://www.protocols.io/view/processing-of-fixed-spheroids-for-tobis-barcoding-c5h3y38n.html

Scientific references demonstrate
the utility of the TissueGrinder technology

The TissueGrinder from Fast Forward Discoveries quite literally saved my project.
I had exhausted all possible options for dissociating fixed cancer cell spheroids for subsequent single-cell signalling studies, when I came across this product. I could hardly believe my eyes when I saw the perfect single-cell suspension generated by my demo TissueGrinder after just 3 minutes of the standard “Harsh” protocol (and with no enzymes!).
It is now an integrated part of my single-cell signalling workflows, and I dare say some of my key discoveries over the last year would not have been possible without this equipment.”
Ralitsa Madsen – UCL, Sir Henry Welcome Postdoctoral Fellow:
https://www.protocols.io/view/processing-of-fixed-spheroids-for-tobis-barcoding-c5h3y38n.html

Generating high quality single cells from tissue samples is a real bottleneck for many applications, such as Flow Cytometry, Single Cell Sequencing, Isolation of Primary Cells, Cancer Cell Line Development to name a few.

Conventional tissue dissociation is is either time-consuming, non-standardized, not scalable or accepting all drawbacks of enzymatic digestion. Or all of them together.

With the TissueGrinder, this bottleneck can be overcome and the door to a new level in single cell analysis can be opened wide.

Application Areas

Accelerate your sample preparation
  • Flow Cytometry
  • Cancer Cell Line Development
  • Isolation of Primary Cells
  • Single Cell Sequencing
  • Single Cell Imaging
  • 3D-Cell-Culture & Cell Printing
  • Tissue Models
  • Drug Screening & Cell-based Assays
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Application Areas

Accelerate your sample preparation
  • Flow Cytometry
  • Cancer Cell Line Development
  • Isolation of Primary Cells
  • Single Cell Sequencing
  • Single Cell Imaging
  • 3D-Cell-Culture & Cell Printing
  • Tissue Models
  • Drug Screening & Cell-based Assays

Conv. Technology A

20 min processing time
  • Enzyme-free
  • Single Cell Quality
  • Integrated Filtration
  • Sterility
  • Integrated Process Control
  • Grinding Slots

Conv. Technology B

> 60 min processing time
  • Enzyme-free
  • Single Cell Quality
  • Integrated Filtration
  • Sterility
  • Integrated Process Control
  • Grinding Slots

TissueGrinder

< 5 min processing time
  • Enzyme-free
  • Single Cell Quality
  • Integrated Filtration
  • Sterility
  • Integrated Process Control
  • Grinding Slots

Conv. Technology A

20 min processing time
  • Enzyme-free
  • Single Cell Quality
  • Integrated Filtration
  • Sterility
  • Integrated Process Control
  • Grinding Slots

TissueGrinder

< 5 min processing time
  • Enzyme-free
  • Single Cell Quality
  • Integrated Filtration
  • Sterility
  • Integrated Process Control
  • Grinding Slots

Conv. Technology B

> 60 min processing time
  • Enzyme-free
  • Single Cell Quality
  • Integrated Filtration
  • Sterility
  • Integrated Process Control
  • Grinding Slots

Fast Forward Discoveries GmbH

Theodor-Kutzer-Ufer 1-3
CUBEX41
68167 Mannheim
Germany

CUBEX41

Altenhöferallee 3
FIZ
60438 Frankfurt/Main
Germany

FIZ - Frankfurter Innovationszentrum Biotechnologie

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