Hepatocytes directly isolated from liver tissue show a functionality very similar in culture as in vivo. They have become the “gold standard” for metabolism, clearance, hepatoxicity and drug-drug interaction and a lot more. The development of various mouse models have increased the availability of tissue samples over the past two decades.
With the FFX TissueGrinder, liver samples can now be dissociated into single cell suspensions within a matter of just a few minutes enzyme-free. It is therefore a superior new option for obtaining a high yield of premium viable cells maintaining surface markers.
In a flow cytometry scatterplot, hepatocytes are easily visible, when the tissue sample is dissociated with the TissueGrinder.
In contrast, hepatocytes cannot be analyzed with enzymatic tissue dissociation.
The TissueGrinder gives a good yield of hepatocytes, while there are close to none retrievable from the enzymatic process.
The generated primary cells can be employed to create tissue models such as spheroids, micro tissues, organoids and cell printed systems. New cell lines can easily be established for molecular cell characterization and drug screenings.
Sounds too good to be true?
Then contact us to arrange a test run with your own samples!