Fast & Label-Free Deformability Cytometry of Tissue with TissueGrinder

Here, we present a rapid and label-free diagnostic method for solid tissue biopsies. The approach combines the enzyme-free, mechanical dissociation of tissues using a tissue grinder for the quick and simple isolation of viable, single cells with the sequential assessment of cellular physical phenotypes of thousands of individual cells using Real Time-Fluorescence Deformability Cytometry (RT-FDC).

Microfluidic Chip in Deformability Cytometry Device © Rivercyte 2023

Our findings demonstrate that the TissueGrinder can enable new diagnostic potential using established and novel analytical methods:

The method delivers results within 30 minutes, providing the basis for a fast and marker-free diagnostic pipeline to detect pathological changes in solid biopsies.

Single-cell suspensions obtained by enzyme-free mechanical dissociation are more representative of the actual cell populations in tissue and, thus, perfect for an unbiased examination of the cellular landscape.

Rapid and unbiased analysis of disease state in inflammation and malignancy is now possible.

Reference: Soteriou, Kubánková et al., 2023, Rapid single-cell physical phenotyping of mechanically dissociated tissue biopsies. Nature Biomedical Engineering https://www.nature.com/articles/s41551-023-01015-3

By enzymatic dissociation, some cell populations can be lost, or cell properties altered. In contrast, our TissueGrinder makes all cells accessible for flow cytometry or other analysis.

This is the basis for pioneering research discoveries and enables new approaches in personalized medicine.

TissueGrinder

enzymatic

Sounds too good to be true?

Then contact us to arrange a test run with your own samples!