Here, we present a rapid and label-free diagnostic method for solid tissue biopsies. The approach combines the enzyme-free, mechanical dissociation of tissues using a tissue grinder for the quick and simple isolation of viable, single cells with the sequential assessment of cellular physical phenotypes of thousands of individual cells using Real Time-Fluorescence Deformability Cytometry (RT-FDC).
Microfluidic Chip in Deformability Cytometry Device © Rivercyte 2023
Our findings demonstrate that the TissueGrinder can enable new diagnostic potential using established and novel analytical methods:
Reference: Soteriou, Kubánková et al., 2023, Rapid single-cell physical phenotyping of mechanically dissociated tissue biopsies. Nature Biomedical Engineering https://www.nature.com/articles/s41551-023-01015-3
By enzymatic dissociation, some cell populations can be lost, or cell properties altered. In contrast, our TissueGrinder makes all cells accessible for flow cytometry or other analysis.
This is the basis for pioneering research discoveries and enables new approaches in personalized medicine.
Sounds too good to be true?
Then contact us to arrange a test run with your own samples!